On campus laboratories allow fundamental and basic science research to investigate mechanisms underlying health, and the neurophysiology, prevention and pain treatment methods offered to the public. Collaborative arrangements with individual university researchers have also enabled some CMCC research faculty to participate in studies in laboratories outside of CMCC.
Clinical studies seeking to improve the quality of patient life are carried out in the outpatient clinic, rehabilitation, and radiology departments. The Department of Rehabilitative and Assessment Services (DRAS) provides for clinical studies of spinal pain and injury with equipment for human performance testing.
Immunology and Cell Biology Lab
CMCC’s state-of the art immunology laboratory (>600 ft2) encompasses a media preparation area, general workspace with 6 laboratory benches, a separate tissue culture room and a data processing room. This space can accommodate 6-8 researchers performing independent experimental procedures. Physical facilities include a chemical hood, 4 sinks, 110 and 220 V power supply, telephone and Internet access lines.
Major equipment in the immunology lab includes:
- Biological safety cabinet (FORMA Laminar Flow Hood): tissue culture room
- Refrigerated centrifuge (Beckman, Allegra X-22R): tissue culture room
- Air-jacketed FORMA incubator with CO2 tanks: tissue culture room
- Refrigerators: 1 in tissue culture room, 1 (Sanyo Medicool, with -30C freezer): main area
- Deep (-78C) FORMA freezer: main area
- VWR Steam sterilizer: main area
- Streem II water distiller: main area
- Eppendorff high speed microcentrifuge: main area
- Beckman Coulter Z2 Counter: main area
- BIO-TEK multichannel microplate reader
- Electronic balances (Precision 320, Sartorius): main area
- pH-meters: main area
- Microscopes (4): Leica precision with Sony monitor: tissue culture room, 3 in main area including inverted (Olympus) microscope
- Eppendorf BioPhotometer Plus: main area
- Titer plate shaker: main area
- Leica DM LB2 light microscopy with Nikkon DS Fi1 Digital; Sight Apparatus Camera Device
- Leica RM 2125 RT Microtome
- Leica EG 1150C Cooling Plate
- Parrafin Dispenser
- Sanyo Drying Oven
- Biorad Tetracell Western Blot Apparatus
Smaller equipment housed in the lab include: precision micropipets (single and multichannel), plate heaters, magnetic stirrers, water baths, vacuum pump, magnetic separator, electrophoresis equipment and other standard “wet” biological laboratory equipment .
There are currently 2 computers in the lab. The main area computer provides operating systems for BIO-TEK microplate reader and Beckman Coulter Counter. The second computer, located in the data processing room (Windows XP) is used for word processing and statistical functions. Internet access is available on both units and a full time IT department provides all necessary equipment maintenance and services.
A 600 foot2 space attached to the Rehabilitation center for human performance testing (total space ~800 ft2) is adjacent to the outpatient clinic on the main floor of CMCC. The lab is equipped with capacity for human performance testing, monitoring kinematics (optoelectronic & electromagnetic), eight channels of myoelectric signal capture, accelerometry, single axis force measures and a 6 DOF (3 forces, 3 moments) force plate and isolation transformers, pressure sensing mats, a research Ultrasonix ultrasound testing system, GaitRite gait monitoring system and gait (AMTI) runway.
All measures can be recorded simultaneously using a Motion Monitor system. New and custom sensors can be added to Motion Monitor or run on a separate computer running Labview. Post processing of results can be done using Matlab. All measures can be time-linked and captured simultaneously through a multiplexing system. A backlighted digitizing system is available for AutoCad design and radiographic mensuration purposes. An imaging ultrasound is available for research. The ultrasound has the ability to control various image properties in order obtain the most suitable image.
- Ultrasound Imaging Unit; Ultrasonix
- Electromyography (EMG) recording equipment ; BIOPAC
- Position/Kinematic sensors; NDI Optotrak Certus and Polhemus Liberty
- Pressure Mats; Gait-Rite, Tekscan Conformat
- Force-plates; AMTI
- Loadcells; Futek
- Accelerometers; Bruel & Kjaer
- Matlab Modeling Software
- Anybody Dynamic Software
Machining and Fabrication Lab
Specialized parts needed for research that cannot be purchased are made in the Fabrication Shop. Materials such as wood, metal, plastics are available in the shop.
- Table Saw
- Drill Press
- Band Saw
- Chop Saw
- Metal Lathe
Small Animals and Material Testing Laboratory
Research in this laboratory helps us understand what happens when the spine gives way, why tissues respond differently from treatment to treatment, how the force from manual treatment spreads through tissues, and how we can make treatment work better.
Small Animals and Materials Testing Laboratory Equipment:
- Mechanical Testing System (MTS); McMesin
- -78 C Deep Freezer
An MTS is available to perform mechanical properties testing of materials. A deep freezer can be used to store some biological materials.
CMCC’s new neurophysiology laboratory is designed for physiological studies on animal models of neurological and spinal diseases. Current studies include investigations of the effects of physical therapies on spinal cord blood flow, and an investigation of the sensory innervation of the vertebral arteries. As such, the laboratory consists of a small secure surgical room (<250 ft2) with all of the equipment needed to perform small animal surgery. This equipment includes:
- CWE SAR-830P small animal ventilator
- CWE Gemini respiratory gas analyzer
- Physiotemp TCAT-2LV temperature controller and heating pad
- Zeiss binocular surgical microscope
- Dolan-Jenner MI-150 high intensity illuminator
- Kopf stereotaxic unit
- Narishige micromanipulators
Experimental data is processed through a data acquisition system consisting of:
- MacBook Pro computer
- ADInstruments Powerlab 8/30
- Animal and Human EKG bioamplifiers
- EKG bioamplifier
- Blood flowmeter and Doppler laser probes
- Neuroamp and headpieces for microneurography
- Stimulus Isolator
- 2 multipurpose bridge amps
The laboratory also has a:
- Nikon SMZ binocular dissecting microscope
- Complete set of surgical instruments
- Coarse and fine weighing scales
- Perfusion system for tissue preservation
- Fume hood
- Laboratory refrigerator
The neurophysiology laboratory operates under a certificate of registration of a research facility issued by the Ontario Ministry of Agriculture, Food and Rural Affairs. CMCC currently has no housing facilities for small animals. In order to ensure optimal animal care, experimental animals are housed off-campus and delivered on a just-in-time basis as needed for studies.
Human Gross Anatomy Laboratory
The Anatomy facilities include: Human Gross Anatomy Laboratory; body receiving area; cold storage; anatomy preparation room; bone library; anatomy technician/clerk office; histology laboratory; the John Duckworth Memorial Museum of Anatomy; and four offices for full time faculty.
The anatomy laboratory is provided with equipment necessary for the dissection of human cadavers: there are 18 dissection tables with embalmed cadavers and a number of gross anatomy and neuroanatomy prosections, disarticulated and fully articulated skeletons. Each table is equipped with a Dissection Guide, Laboratory objectives, and an Atlas of Human Anatomy (Grant’s or Netter’s). There are surgical lamps, similar to those used in dental offices, installed directly above each table. There are 18 dissection tool kits, containers with the wetting solution to properly maintain the cadavers, and various other tools, including a striker saw, safety glasses, masks, and gloves.
X-ray view boxes are provided in the lab to observe X-Ray films. The body receiving area is equipped with a lift to receive and transport the bodies. The area is adequately constructed and provides volatile storage for related chemicals: glycol, methanol, alcohol. It is equipped with gas and vapour detectors, and an anti-spark light switch. There is an adjacent cold storage area that is used to freeze and store cadavers and specimens.
The anatomy preparation room is used for embalming cadavers, post-embalming perfusion with Infutrace to limit exposure to hazardous vapours, latex injections. Additionally, body washing and wrapping procedures take place in this area. The room is equipped with a specially manufactured embalming table, three embalming pumps, storage shelves, lift truck, a dehumidifier to prevent condensation build up on the floor, dissection tools, labelled chemicals, a bulletin board to display the list of chemicals used, Material Safety Data Sheets with first aid, and instructions on the cadaver embalming procedure.
Chiropractors are familiar with the use of cadavers for learning and research in anatomy and neural sciences. CMCC is one of only nine laboratories in Ontario that oversee body donation programs, which is provincially controlled by the Chief Coroner’s Office and regulated by the Anatomy Act of Ontario. The anatomy lab is also home to the only anatomy museum in Canada that is specific to neuromusculoskeletal issues. Most of the museum specimens were prepared by CMCC anatomy faculty and students. Learn more about the CMCC Body Donation Program.
Offices in the Graduate Education and Research Programs (GERP) area
Studies on education research and health services/policy are supported through the offices and information technology capacity of Graduate Education and Research.
Collaborative arrangements with individual university researchers in basic science, clinical studies and health care policy research have enabled CMCC research faculty, residents and students to participate in studies in laboratories and clinics at the University of Toronto, McMaster University, the Université du Québec à Trois-Rivières, the University of Calgary, University of Waterloo, St. Michael’s Hospital and the Ontario Ministry of Health and Long-Term Care. International collaborations include those with other chiropractic colleges, the University of Vermont, Columbia University, the Texas Back Institute and others.